Expression of the eicosapentaenoic acid synthesis gene cluster from Shewanella sp. in a transgenic marine cyanobacterium, Synechococcus sp.
نویسندگان
چکیده
منابع مشابه
Draft Genome Sequence of Marine Cyanobacterium Synechococcus sp. Strain NKBG15041c
Synechococcus sp. strain NKBG15041c was isolated as a fast-growing marine cyanobacterium. Genetic transformation techniques using this strain have been well established for metabolic engineering. Here we report the draft genome sequence for this strain, consisting of 44 contigs containing a total of 3,180,043 bp and 3,224 putative protein-coding genes.
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An eicosapentaenoic acid-producing bacterium, previously described as Shewanella sp. strain SCRC-2738, was classified by phenotypic characterization, chemotaxonomic analysis, 16S rRNA gene sequence analysis and DNA-DNA hybridization. The isolate was Gram-negative, rod-shaped and motile by using polar flagella. The strain grew at 4-32 degrees C; the optimum growth temperature was 27 degrees C. N...
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The application of synthetic biology requires characterized tools to precisely control gene expression. This toolbox of genetic parts previously did not exist for the industrially promising cyanobacterium, Synechococcus sp. strain PCC 7002. To address this gap, two orthogonal constitutive promoter libraries, one based on a cyanobacterial promoter and the other ported from Escherichia coli, were...
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We describe a novel mechanism of site-specific recombination in the unicellular marine cyanobacterium Synechococcus sp. PCC7002. The specific recombination sites on the smallest plasmid pAQ1 were localized by studying the properties of pAQ1-derived shuttle-vectors. We found that a palindromic element, the core sequence of which is G(G/A)CGATCGCC, functions as a resolution site for site-specific...
متن کاملMolecular cloning and characterization of the recA gene from the cyanobacterium Synechococcus sp. strain PCC 7002.
The recA gene of Synechococcus sp. strain PCC 7002 was detected and cloned from a lambda gtwes genomic library by heterologous hybridization by using a gene-internal fragment of the Escherichia coli recA gene as the probe. The gene encodes a 38-kilodalton polypeptide which is antigenically related to the RecA protein of E. coli. The nucleotide sequence of a portion of the gene was determined. T...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1997
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-143-8-2725